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Emissive base analogs are powerful tools for probing nucleic acids at the molecular level.

Herein we describe the development and thorough characterization of Exceptionally bright adenine pAa versatile base analog with exceptional fluorescence properties.

When Exceptionally bright into DNA, pA pairs selectively with thymine without perturbing the B-form structure and is among the brightest nucleobase analogs reported so far.

The high brightness at emission wavelengths above nm also makes it suitable for fluorescence microscopy, as demonstrated by imaging of single liposomal constructs coated with cholesterol-anchored pA—dsDNA, using total internal reflection fluorescence microscopy. Exceptionally bright, pA is Exceptionally bright highly promising for two-photon excitation at nm, with a brightness 5.

The article was received on 23 Decaccepted on 01 Mar and first published on 01 Mar This article is licensed under a Creative Commons Attribution 3. Exceptionally bright from this article can be used in other publications provided that the Exceptionally bright acknowledgement is given with the reproduced material.

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Exceptionally bright to tab navigation Download options Please wait Supplementary information PDF K. For reproduction of Exceptionally bright from NJC: For reproduction of material from PCCP: For reproduction of material from PPS: For reproduction of material from all other RSC journals: Search articles by author Mattias Bood.

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